Reviews
This is an excellent and well-executed replication study that strengthens confidence in the reproducibility of the original findings on Protein Y’s role in oxidative stress. The methodology is rigorous, and the results align closely with the original study. My only recommendation is adding a brief discussion to address variability across replicates. This is a minor point that will further enhance the clarity and transparency of the report.
The report provides robust validation of the original study, with consistent findings and transparent data presentation. Nonetheless, I would like the inclusion of raw data and statistical analyses, which add to the report’s credibility. Additionally, I recommend expanding the Figure 2 legend to define how biological replicates were derived. Overall, I support the acceptance of this report with these minor clarifications.
I would like to congratulate the authors for this report with a strong alignment with the original findings and its methodological rigor. The study successfully reproduces key results, confirming Protein Y’s role in regulating oxidative stress and mitochondrial function. To make the results more robust, I suggest including a brief description in the Methods section about how siRNA transfection efficiency was validated. This minor suggestion will further strengthen the report, which is otherwise ready for acceptance.
Response from the authors
We thank the reviewers for their constructive feedback, which has been fully addressed in the revised manuscript. To address Reviewer ID01’s concerns, we added a discussion about variability across replicates, noting consistent cell handling and experimental conditions. In response to Reviewer ID02, we included a link to the raw data and code used for statistical analyses in the ‘Additional information’ section. We also updated Figure 2 legend to define biological replicates as derived from three independent cell isolations. Finally, to address Reviewer ID03’s comments, we included a statement in the Methods section confirming that siRNA transfection efficiency was validated using fluorescently labelled siRNA. These revisions ensure clarity, robustness, and alignment with the reviewers’ suggestions.
