Our replication study focused on validating Figures 2 of the original article, which investigated the role of Protein Y in oxidative stress responses. The experiments were conducted under the same conditions described in the original study, using mouse embryonic fibroblasts (MEFs). Below, we detail our findings, which corroborate the conclusions of the original work.
Cell viability was assessed using the MTT assay to determine the impact of Protein Y knockdown under oxidative stress conditions. Consistent with the original findings, knockdown of Protein Y led to a significant reduction in cell viability. Specifically, knockdown cells exhibited a 35% decrease in viability compared to controls exposed to scrambled siRNA, closely aligning with the 33% reduction reported in the original study (p = 0.003). These results, shown in Figure 2, were consistent across three biological replicates, with mean viability values of 65% ± 3% for knockdown cells and 100% ± 4% for control cells. This finding underscores the critical role of Protein Y in maintaining cellular viability under oxidative conditions.
The consistency of these results across three independent replicates strengthens the reproducibility of the original study’s conclusions.